Onion cells

Real Lab Procedure

1.Cut open an onion

2.Use forceps to peel a thin layer of epidermis from the inside

3.Lay the layer of epidermis on a microscope slide

4.Add a drop of iodine/methylene solution to the layer

5.Carefully place a cover slip over the layer


There are a large number of regularly shaped cells lying side by side and each cell has a distinct cell wall.

A distinct nucleus is present on the periphery of each cell.

A large vacuole is present at the centre of each cell, and is surrounded by the cytoplasm.


As cell walls and large vacuoles are clearly observed in all the cells, the cells placed for observation are plant cells.


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