Mitosis is a process where a single cell divides into two identical daughter cells. To see mitosis in action one needs to look at living cells. This could be examined by the following experiment.
- Cut off 1-2 cm of the root tips. Put in a small volume of ethanoic acid on a watch glass
- Meanwhile, heat 10-25 cm3 of 1 M hydrochloric acid to 60 °C in a water bath
- Wash the root tips in cold water for 4-5 minutes and dry on filter paper
- Use a mounted needle to transfer the root tips to the hot hydrochloric acid and leave for 5 minutes.
- Wash the root tips again in cold water for 4-5 minutes and dry on filter paper
- Use the mounted needle to remove two root tips onto a clean microscope slide
- Cut each about 2 mm from the growing root tip. Discard the rest, but keep the tips
- Add a small drop of stain and leave for 2 minutes
- Break up the tissue with scissors and add a drop of water
- Cover with a coverslip and squash
-View the root tips under a microscope and look for the chromosomes within cells which are actively dividing
-Observe the different stages of Mitosis